Chick embryo developmental stages 1. YO D EVEl.O IV! E ‘lTAL ST/ -XGES 2. CHICKEN EGG ANATOMY Blastoderrn Fibmus lave’ Lateb.
In this article we will discuss about the development stage of chick embryo, fertilized eggs are procured from recognised poultry farm and incubated in the laboratory.
What you can see here are small 'postage-stamp' versions of these movies of serially sectioned frog and chick embryos. All show sequential cross sections going anterior-to-posterior (head-to-tail). Get Quicktime Serially Sectioned Frog Embryo Movies 4 mm 5 mm 7 mm 10 mm Serially Sectioned Chick Embryo Movies 24 hr 33 hr 33 hr 48 hr 48 hr 56 hr. CHICK EMBRYO AT 24 HOURS. Incubation period the chick embryo is oval in shape. The primitive streak is seen. From the hensen's node a notochord is developed. Chick embryo four pairs of somities are present. NEURALFOLDS: On either side of mid dorsal line the ectoderm becomes thick. It forms longitudinal folds. Download this stock image:. A laboratory manual and text-book of embryology. CHICK EMBRYO OF EIGHTEEN PRIMITIVE SEGMENTS 61 Transverse Section through the Primitive (Hensen's) Node or Knot (Fig. 40).—The section shows the three germ layers bound together at the "knot" or node into a mass of undifferentiated tissue. The mesoderm is split laterally into the somatic.
1. Chick: M. 4 Hours of Incubation:
1. Four hours after incubation of the egg shows differentiation of the blastodisc into area pellucida and area opaca. (Fig. 7).
2. One quadrant of area pelluciada becomes thickened, which marks the future caudal end of embryo.
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3. After 7 to 8 hours, the thickening becomes more elongated and represented start of primitive streak.
2. Chick: W.M. 16 Hours Embryo:
Comments:
1. 16 hours after incubation the primitive streak becomes so distinct that embryos are characterized as being in primitive streak stage. (Fig. 8).
2. In fixed and stained slide, w.m. is composed of central furrow, called as primitive groove lined by thickened primitive ridges.
3. At the cephalic end of the primitive streak, closely-packed cells form thickened area, called as Hensen’s node. Part of area pellucida adjacent to the primitive streak shows increased thickness and forms embryonic elliptical shape.
4. Area pellucida assumes elliptical shape.
5. Elongated primitive streak represents long axis of future embryonic body.
6. Caudal end of the streak is that which lies close to the area opaca.
3. W.M. 18 Hours Chick Embryo:
1. It is a W.M. of 18 hours stage of chick embryo.
2. At this stage the dark peripheral area opaca and central translucent area pellucida are distinctly visible.
3. In the anterior part is present the pro-amnion, which is a small and comparatively more translucent region of area pellucida and is characterised by the absence of mesoderm.
4. In the middle of area pellucida, in the posterior half, runs a primitive streak having a primitive groove through its centre. The primitive groove is being bound by primitive folds.
5. In the anterior half of area pellucida, in the middle, runs a neural groove bound by neural folds.
6. The primitive streak and neural groove is separated by a thickening-the Hensen’s node having a small depression in the centre-the Hensen’s pit.
7. The primitive streak gives rise to an out-growth, the notochord immediately below the primitive groove.
4. W.M. of 21 Hours Chick Embryo:
1. It is a W.M. of 21 hours chick embryo.
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2. At this stage the dark peripheral area opaca and central translucent and colourless area pellucida are distinctly visible.
3. In the anterior part are present the pro-anmnion, which is a small and comparatively more translucent region of area pellucida and is characterised by the absence of mesoderm.
4. In the middle of area pellucida, in the posterior half, runs a primitive streak having a primitive groove through its centre. The primitive groove is being bound by primitive folds.
5. In the anterior half of area pellucida, in the middle, runs a neural groove bound by neural folds.
6. The primitive streak and neural groove are separated by a thickening, the Hensen’s nod having a small depression in the centre of the Hensen’s pit.
7. The primitive streak gives rise to a small outgrowth, the notochord immediately below the primitive groove and to mesoderm on either side.
8. At this stage embryonic and extra ambryonic regions have also become distinguished in the area pellucida.
9. In the anterior most part the ectoderm has given rise to head fold, which is a pocket-like extension of neural folds.
10. With the ectoderm the underlying endoderm is also transformed into a pocket-like structure the -foregut.
11. The proambion is comparatively reduced in size.
5. W.M. of 24 Hours or 4 Pairs of Somites Stage of Chick Embryo:
1. It is a W.M. of 24 hours 4 pairs of somites stage of chick embryo.
2. At this stage the dark peripheral area opaca and central translucent and colourless area pellucida are distinctly visible.
3. In the anterior part is present the proamnion, which is a small and comparatively more translucent region of area pellucida and is characterised by the absence of mesoderm.
4. In the middle of area pellucida, in its posterior half runs a primitive streak with a primitive groove in its centre. The primitive groove is bound by primitive folds.
5. In the anterior half of area pellucida, in the middle, runs the neural groove bound by neural folds.
6. The primitive streak and neural groove are separated by Hensen’s node having a small depression in the centre-the Hensen’s pit.
7. Immediately below the primitive groove the primitive streak gives rise to a small out- growth, the notochord and on either side to mesoderm.
8. In the area pellucida embryonic and extra embryonic regions also become distinguished.
9. In the anterior- most part the ectoderm has given rise to head fold, which is a pocket-like extension of neural folds. The underlying endoderm is also transformed into a pocket-like foregut. The proamnion is greatly reduced.
10. In front of Hensen’s node the mesoderm of embryonic area differentiated into 3-4 pairs of mesodermal somites.
11. The neural canal, in the region of head fold, gives rise to forebrain.
12. The foregut extends on either side into an amino-cardiac vesicle.
6. W.M. of 30 Hours of 8-10 Pairs of Somites Chick Embryo:
1. It is W.M. of 30 hours of chick embryo or 8-10 pairs of somite stage of chick embryo.
2. At this stage the dark peripheral area opaca and central translucent and clourless area pellucida are distinctly visible.
3. In the anterior part is present the proamnion, which is a small and comparatively more translucent region of area pellucida and is characterised by the absence of mesoderm.
4. In the middle of area pellucida, in the posterior half, runs a primitive streak with a primitive groove running through its centre. The primitive groove is bound by primitive folds.
5. In the anterior half of area pellucida, in the middle, runs the neural groove bound by neural folds.
6. The primitive streak and neural groove are separated by Hensen’s node having a small Hensen’s pit in the centre.
7. Immediately below primitive groove the primitive streak gives rise to the notochord and on either side to mesoderm.
8. At this stage embryonic and extra embryonic regions have also become distinguished in the area pellucida.
9. In the anterior-most part, the ectoderm has given rise to head fold which is a pocket like extention of neural folds. The underlying endoderm has transformed into pocket like foregut. The proamnion is reduced.
10. The mesoderm, in front of Hensen’s node, has given rise to 8-10 pairs of somites.
11. In the region of head fold the anterior part of neural canal has given rise to a distinct fore brain.
12. The foregut and cardiac vesicles are sufficiently developed.
13. The extra embryonic area has grown in size.
7. W. M. of 33 Hour Chick Embryo of 11-12 Pairs Somites:
1. It is W.M. of 33 hours chick embryo.
2. At this stage the dark peripheral area opaca and central translucent area pellucida are not distinctly visible.
3. The primitive streak has been comparatively reduced because of great lengthening of neural canal and neural folds.
4. The extra embryonic area has grown in size.
5. The mesoderm, in front of Hensen’s node, has given rise to 11-12 pairs of somites.
6. The foregut and cardiac vesicles are sufficiently developed.
7. The brain is differentiated into fore brain, mid- brain and hind brain.
8. The area opaca has changed into area vasculosa.
9. Proamnion has disappeared.
10. Anterior omphalomesenteric vein has developed.
8. W.M. of Chick Embryo of 13-14 Pairs Somites or 36 Hours:
1. It is W.M. of 36 hours chick embryo.
2. At this stage the dark peripheral area opaca and central translucent and colourless area pellucida are not visible.
3. The extra embryonic area has grown in size.
4. The primitive streak is comparatively reduced because of great lengthening of neural canal and neural folds. The notochord has extended from behind the brain up to the end of body.
5. The mesoderm, in front of Hensen’s node, has given rise to 13-14 pairs of somites.
6. The brain is differentiated into fore brain, mid brain and hind brain.
7. In the fore brain region optic vesicles and in the hind brain region optic vesicles have developed.
8. The area opaca has changed into area vasculosa.
9. Proamnion has disappeared.
10. Anterior omphalomesentric vein and vitelline artery have developed.
11. The cardiac vesicle has given rise to heart.
9. W.M. of 48 Hours Chick Embryo of 26-28 Pairs of Somites:
1. It is W.M. of 48 hours chick embryo.
2. At this stage the area opaca and area pellucida are not visible.
3. The extra embryonic area has grown in size.
4. Primitive streak has disappeared.
5. The mesoderm, in front of Hensen’s node, has given rise to 26-28 pairs of somites.
6. The brain has differentiated into telencephalon, prosencephalon, mesencephalon, metancephalon and mylencephalon.
7. The heart has been differentiated into ventricle and atrium. Sinus venosus and truncusarteriosus have also started developing.
8. The eye has been differentiated into optic cup and lens and optic vesicle has also developed sufficiently.
9. The head region has curved on right side due to cranial flexion.
10. Three pharyngeal gill-slits have also been differentiated.
11. Behind Hensen’ node a tail bud has also developed.
12. Lateral amniotic folds, anterior omphalomesentric vein and vitelline artery have appeared.
10. W.M. of 72 Hours or 36 Pairs of Somites Stage of Chick Embryo:
1. It is W.M. of 72 hours chick embryo.
2. At this stage area opaca and area pellucida are not visible.
3. The extra embryonic area has grown in size.
4. Primitive streak has disappeared.
5. The mesoderm, in front of Hensen’s node, has given rise to 36 pairs of somites.
6. The brain has differentiated into telencephalon, mesencephalon, metancephalon and mylencephalon.
7. The heart has been differentiated into ventricle and atrium.
8. The eye has differentiated into optic cup and lens and optic vesicle has also developed sufficiently.
9. The head region has bent on right side due to cranial flexion.
10. Four pairs of gill-slits have been differentiated.
11. Tail bud is greatly developed and has given rise to allentoic stalk and tail.
12. Lateral amniotic folds, vitelline artery and anterior omphalomesentric vein have developed.
13. In the middle region a pair of fore limb buds and in front of tail a pair of hind limb buds have developed, which will give rise to fore and hind limbs.
14. Olfactory pit, visceral arches, amnion, allantois and amniotic cavity have also developed.
11. W.M. of 96 Hours Chick Embryo:
1. In the chick embryo of 96-hours of incubation, the entire body has been turned through 90 degree and the embryo lies with its left side on the yolk.
2. At the end of 96 hours the body folds have undercut the embryo so that it remains attached to the yolk only by a slender stalk.
3. The yolk salk soon become enclogated, allowing the embryo to become first straight in the mid-dorsal region and then convex dorsally.
4. The progressive increase in the cranial, cervical, dorsal and caudal flexures results in the bending of the embryo on itself so that its originally straight long axis becomes C-shaped and its head and tail lie close together.
5. Optic cup shows the more developed lens.
6. Endo-lymphatic duct arises from the auditory vesicle.
7. Visceral arches have become very much thickened.
72 Hour Chick Embryo
8. Appendage buds increase rapidly in size and become elongated.
9. The number of somites increases to 41 pairs.
10. Allantois has also appeared.
11. Omphalomesenteric artery and omphalomesenteric vein are also developed.
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72 Hour Chick Embryo
Development of early embryo of Frog sections(11 pieces),Development of early embryo of Penaeus orientalis sections (7 pieces),Development of early Embryo of Chicken w.m. & serial transverse sections（24/48/96 hours）.
Torsion: The 24 hours chick embryo is flat. Its ventral surface is in contact with yolk. As the development proceeds the anterior end of embryo is turns towards right and hence the anterior part of embryo comes to lie on the left side of yolk. This twisting is called torsion. At the end of 48 hours chick twisting will reach the cervical flexure. Chick Embryo 48 Hour WM Prepared Microscope Slide Limited Availability of Chick Embryo 48 Hour WM Prepared Microscope Slide. EE8-1 Chick Embryo 48 Hour WM Prepared Microscope Slide Aves 48 hr chick (24-28 somites); wm. A 10% discount applies if you order more than 10 of this item and 15% discount applies if you order more than 25 of this item.
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48 Hour Chick Embryo Whole Mount
Description
1.Product name:Development of early embryo of Frog sections(11 pieces)
2.Type:embryology slides
3.Structure:seeing the frog embryo develepment stage like cleavage,blastula, gastrula,Neural plate stage of egg of Frog under bologcal microscope .
4.Customers:all levels of students/home school programs(school,students,teachers,scientists,Scientific research personne)
5.Material:Extract from fresh material;general glass,blank microscope glass
6.Size:76.2x25.4x1-1.2mm(3'x1') length/width/thickness
7.Packing:we have 6/10/15/25/30/50/100pieces plastic slides box for one set. and 10/12/15/16/26/50/100pieces wooden slides box for one set.
8.Label:We make the label by ourself ! usually in english languages;or at your
requirement(Customer could choose to print labels in any languages)
9.Price:We are direct factory ! Welcome to inquiry !!
Manufacture Process:
Dyeing→Dehydration→Embedding→Sectioning→Unfold→Drying→
Dewaxing→Sealing piece→Test→Drying→Quality inspection
Various embryology prepared slides:
Development of early embryo of Frog sections(11 pieces),Development of early embryo of Penaeus orientalis sections (7 pieces),Development of early Embryo of Chicken w.m. & serial transverse sections（24/48/96 hours）.
48 Hour Chick Embryo Serial Cross Section
Parameter
YHE18001A Development of early embryo of Frog sections(11 pieces)
YHE18001Ⅰ Simple-cell of egg of Frog sec.
YHE18001Ⅱ 2-cell of egg of Frog sec.
YHE18001Ⅲ Early cleavage stage of egg of Frog sec.
YHE18001Ⅳ Late cleavage stage of egg of Frog sec.
YHE18001Ⅴ Early blastula stage of egg of Frog sec.
YHE18001Ⅵ Late blastula stage of egg of Frog sec.
YHE18001Ⅶ Early gastrula stage of egg of Frog sec.
YHE18001Ⅷ Late gastrula stage of egg of Frog sec.
YHE18001Ⅸ Neural plate stage of egg of Frog sec.
YHE18001Ⅹ Neural fold stage of egg of Frog sec.
YHE18001Ⅺ Neural tube stage of egg of Frog sec.
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96 Hour Chick Embryo
Heart formation:
The formation of the heart is mesodermal and arises as two lateral thickenings of the splanchnic mesoderm (primordia) at the level of the head region. These two primordia grow later together in the median plane. Ectoderm (2) and somati mesoderm (4) form the somatopleura; splanchnic mesoderm (5) and endoderm (6) form the splanchnopleura.
1 = Coelome, 2 = Ectoderm, 3 = Neural tube, 4 = Somatic mesoderm, 5 = Splanchnic mesoderm, 6 = Endoderm, 7 = Foregut, 8 = Heart formation, 9 = (Dorsal) mesocardium, 10 = Head mesenchym, 11 = Chorda, 12 = Endocardium, 13 = Epimyocardium, ec = Ectoderm, nb = Neural tube, vd = Foregut, hb =Heart tube, d = Yolk zoom